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bisulfite pyrosequencing primer sets for aire1  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc bisulfite pyrosequencing primer sets for aire1
    Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of <t>AIRE1</t> ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test
    Bisulfite Pyrosequencing Primer Sets For Aire1, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite+pyrosequencing+primer+sets+for+aire1/bisulfite+pyrosequencing+primer+sets+for+aire1/pmc07218560-154-2-3
    Average 90 stars, based on 1 article reviews
    bisulfite pyrosequencing primer sets for aire1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Promoter methylation changes in ALOX12 and AIRE1 : novel epigenetic markers for atherosclerosis"

    Article Title: Promoter methylation changes in ALOX12 and AIRE1 : novel epigenetic markers for atherosclerosis

    Journal: Clinical Epigenetics

    doi: 10.1186/s13148-020-00846-0

    Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of AIRE1 ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test
    Figure Legend Snippet: Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of AIRE1 ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test

    Techniques Used: Methylation, Expressing, Sequencing

    Immunofluorescence staining for AIRE1 and infiltrated CD4(+)-T cells and CD14(+)-monocytes in atherosclerotic plaque and non-plaque intima of common carotid artery of a 54-year-old man. White arrow, cells co-stained with CD14 and AIRE1 antibodies
    Figure Legend Snippet: Immunofluorescence staining for AIRE1 and infiltrated CD4(+)-T cells and CD14(+)-monocytes in atherosclerotic plaque and non-plaque intima of common carotid artery of a 54-year-old man. White arrow, cells co-stained with CD14 and AIRE1 antibodies

    Techniques Used: Immunofluorescence, Staining

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Promoter methylation changes in ALOX12 and AIRE1 : novel epigenetic markers for atherosclerosis
    Article Snippet: USA), FANK1 (Cat. No.: PM00146167), and SERHL2 (Cat. No.: PM00200746); these comprised forward and reverse primer pairs for PCR and a sequencing primer for bisulfite pyrosequencing (Fig. a). .. We used bisulfite pyrosequencing primer sets for AIRE1 (AIRE1-F; biotin-5′-GGGTAGTTTTTGTTAGGGTTTTGA-3′ and AIRE1-R; 5′-TCACCCACCAAAACAACTACCTTA-3′ for PCR and AIRE1-S; 5′-TCAAAAAACTCTCACTTCC-3′ for bisulfite pyrosequencing) and NEOT1 (NEOT1-F; biotin-5′-GTGGGGGGTTTAATTGTAAGAAGT-3′ and NETO1-R; 5′-TCTCTACCTCCCACCCCTTCTTT-3′ for PCR and NETO1-S; 5′-CCCCTTCTTTTCCAT-3′ for bisulfite pyrosequencing) (Fig. a). .. PCR for bisulfite pyrosequencing of each gene was performed in a total volume of 50 μL containing 1× PCR buffer, 0.2 mmol/L dNTP, 1 unit of Taq polymerase, 1 μL (15 ng) of bisulfite-treated DNA, and 0.1 mmol/L of forward and reverse primers for individual genes of which one primer for each gene was biotinylated at the 5′ end.



    Similar Products

    bisulfite pyrosequencing primer sets for aire1  (Pyrosequencing Inc)
    90
    Pyrosequencing Inc bisulfite pyrosequencing primer sets for aire1
    Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of <t>AIRE1</t> ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test
    Bisulfite Pyrosequencing Primer Sets For Aire1, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite+pyrosequencing+primer+sets+for+aire1/bisulfite+pyrosequencing+primer+sets+for+aire1/pmc07218560-154-2-3
    Average 90 stars, based on 1 article reviews
    bisulfite pyrosequencing primer sets for aire1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of AIRE1 ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test

    Journal: Clinical Epigenetics

    Article Title: Promoter methylation changes in ALOX12 and AIRE1 : novel epigenetic markers for atherosclerosis

    doi: 10.1186/s13148-020-00846-0

    Figure Lengend Snippet: Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR ( a ) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers ( b ). Correlation between promoter methylation and expression of AIRE1 ( c ) and ALOX12 ( d ) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, * p < 0.05 on paired t test

    Article Snippet: We used bisulfite pyrosequencing primer sets for AIRE1 (AIRE1-F; biotin-5′-GGGTAGTTTTTGTTAGGGTTTTGA-3′ and AIRE1-R; 5′-TCACCCACCAAAACAACTACCTTA-3′ for PCR and AIRE1-S; 5′-TCAAAAAACTCTCACTTCC-3′ for bisulfite pyrosequencing) and NEOT1 (NEOT1-F; biotin-5′-GTGGGGGGTTTAATTGTAAGAAGT-3′ and NETO1-R; 5′-TCTCTACCTCCCACCCCTTCTTT-3′ for PCR and NETO1-S; 5′-CCCCTTCTTTTCCAT-3′ for bisulfite pyrosequencing) (Fig. a).

    Techniques: Methylation, Expressing, Sequencing

    Immunofluorescence staining for AIRE1 and infiltrated CD4(+)-T cells and CD14(+)-monocytes in atherosclerotic plaque and non-plaque intima of common carotid artery of a 54-year-old man. White arrow, cells co-stained with CD14 and AIRE1 antibodies

    Journal: Clinical Epigenetics

    Article Title: Promoter methylation changes in ALOX12 and AIRE1 : novel epigenetic markers for atherosclerosis

    doi: 10.1186/s13148-020-00846-0

    Figure Lengend Snippet: Immunofluorescence staining for AIRE1 and infiltrated CD4(+)-T cells and CD14(+)-monocytes in atherosclerotic plaque and non-plaque intima of common carotid artery of a 54-year-old man. White arrow, cells co-stained with CD14 and AIRE1 antibodies

    Article Snippet: We used bisulfite pyrosequencing primer sets for AIRE1 (AIRE1-F; biotin-5′-GGGTAGTTTTTGTTAGGGTTTTGA-3′ and AIRE1-R; 5′-TCACCCACCAAAACAACTACCTTA-3′ for PCR and AIRE1-S; 5′-TCAAAAAACTCTCACTTCC-3′ for bisulfite pyrosequencing) and NEOT1 (NEOT1-F; biotin-5′-GTGGGGGGTTTAATTGTAAGAAGT-3′ and NETO1-R; 5′-TCTCTACCTCCCACCCCTTCTTT-3′ for PCR and NETO1-S; 5′-CCCCTTCTTTTCCAT-3′ for bisulfite pyrosequencing) (Fig. a).

    Techniques: Immunofluorescence, Staining